Genomics

desert_tortoise

The biological sciences have undergone a revolution in the past decade with the the development of next-generation DNA sequencing technologies and advances in bioinformatic analysis. This is fundamentally changing the way in which we carry out research. It is now possible to generate genomes for a number of different species to address a developmental or evolutionary biological question. Genetic disorders can be studied by comparing whole exome sequences of a number of affected individuals. The process of regeneration can be analyzed at the transcriptome level, so that the expression of all genes can be assessed at the same time. Genomic and bioinformatic approaches have been at the core of projects in the Kusumi Lab, including:

  • Transcriptomic analysis of somite-stage embryos in the green anole lizard (Anolis carolinensis), Eckalbar et al., 2012
  • Genome reannotation of the green anole, Eckalbar et al., 2013
  • Identification of differentially expressed genes in tail regeneration in the green anole, Hutchins et al., 2014
  • Analysis of microRNA and proteomic expression in tail regeneration of the green anole
  • Comparative evolutionary genomic analysis of diversity in anole lizards: Anolis auratus, A. apletophallus, and A. frenatus
  • Genome sequencing of the Mohave desert tortoise, Gopherus agassizii, for conservation genetics.